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How Many Pokémon Can You Get with Only Pokémon Ruby?

Although the game contains all 386 Pokémon that existed at the time of the game's release, the player can only obtain Pokémon that are part of the Hoenn Pokédex without trading. The Hoenn Pokédex contains 200 Pokémon (202 with Jirachi and Deoxys), meaning a total of 184 Pokémon from the National Dex cannot be obtained. Those 184 are not listed on this page; check out the Locations page to see which Pokémon are excluded.

The number of Pokémon in the Hoenn Pokédex that are available without trading is 177. The list of Pokémon you cannot get without trading, which equals 25 species, is as follows:

Two Starters (6) - 6 total
As you can only select one starter Pokémon, the other two starters, and their evolutions, are unavailable; this totals 6 Pokémon.
Trade Evolutions (6) - 12 total
Golem, Machamp, Alakazam, Kingdra, Huntail, and Gorebyss evolve by trade. Without the ability to trade, they are unobtainable.
One Fossil Pokémon (2) - 14 total
As you can only obtain one Fossil on Route 111, you miss out on the other Fossil Pokémon (Lileep or Anorith) and its evolution.
Version Exclusives (8) - 22 total
The following Pokémon cannot be obtained without the use of glitches, trading, or events: Lotad, Lombre, Ludicolo, Sableye, Seviper, Lunatone, Latias, and Kyogre.
Moon Stone Evolutions (1) - 23 total
Ruby players can only get one Moon Stone, but two Pokémon require it: Delcatty and Wigglytuff. One of these Pokémon is therefore unobtainable.
Mythical/Event (2) - 25 total
Outside of glitches, Jirachi and Deoxys were only obtainable via events that are no longer active, or in Jirachi's case via the Colosseum Bonus Disc (which requires trading).

The Professor Oak challenge guide for Ruby can be found here, although I've made a shorter list of encounters below in case anything happens to the external guide.

Professor Oak Challenge Notes

Pre-Badge 1 (Roxanne) - 37 Total Pokémon

Badge 1 through Badge 2 (Wattson) - 67 Total Pokémon

Badge 2 through Badge 3 (Flannery) - 93 Total Pokémon

Badge 3 through Badge 5 (Norman) - 102 Total Pokémon

Badges 5 through 6 (Tate & Liza) - 159 Total Pokémon

Badges 6 through 7 (Wallace) - 168 Total Pokémon

Badges 7 through 8 (Winona) - 171 Total Pokémon

Badge 8 through Elite Four - 172 Total Pokémon

Post-Elite Four - 177 Total Pokémon