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How Many Pokémon Can You Get with Only Pokémon Sapphire?

Although the game contains all 386 Pokémon that existed at the time of the game's release, the player can only obtain Pokémon that are part of the Hoenn Pokédex without trading. The Hoenn Pokédex contains 200 Pokémon (202 with Jirachi and Deoxys), meaning a total of 184 Pokémon from the National Dex cannot be obtained. Those 184 are not listed on this page; check out the Locations page to see which Pokémon are excluded.

The number of Pokémon in the Hoenn Pokédex that are available without trading is 178. The list of Pokémon you cannot get without trading, which equals 24 species, is as follows:

Two Starters (6) - 6 total
As you can only select one starter Pokémon, the other two starters, and their evolutions, are unavailable; this totals 6 Pokémon.
Trade Evolutions (6) - 12 total
Golem, Machamp, Alakazam, Kingdra, Huntail, and Gorebyss evolve by trade. Without the ability to trade, they are unobtainable.
One Fossil Pokémon (2) - 14 total
As you can only obtain one Fossil on Route 111, you miss out on the other Fossil Pokémon (Lileep or Anorith) and its evolution.
Version Exclusives (8) - 22 total
The following Pokémon cannot be obtained without the use of glitches, trading, or events: Seedot, Nuzleaf, Shiftry, Mawile, Zangoose, Solrock, Latios, and Groudon.
Mythical/Event (2) - 24 total
Outside of glitches, Jirachi and Deoxys were only obtainable via events that are no longer active, or in Jirachi's case via the Colosseum Bonus Disc (which requires trading).

The Professor Oak challenge guide for Sapphire can be found here, although I've made a shorter list of encounters below in case anything happens to the external guide.

Professor Oak Challenge Notes

Pre-Badge 1 (Roxanne) - 37 Total Pokémon

Badge 1 through Badge 2 (Wattson) - 67 Total Pokémon

Badge 2 through Badge 3 (Flannery) - 92 Total Pokémon

Badge 3 through Badge 5 (Norman) - 101 Total Pokémon

Badges 5 through 6 (Tate & Liza) - 160 Total Pokémon

Badges 6 through 7 (Wallace) - 169 Total Pokémon

Badges 7 through 8 (Winona) - 172 Total Pokémon

Badge 8 through Elite Four - 173 Total Pokémon

Post-Elite Four - 178 Total Pokémon